Small molecule analysis using laser desorption/ionization mass spectrometry on nano-coated silicon with self-assembled monolayers |
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Authors: | Ö mü r Ç elikbıç ak,Gö khan Demirel,Erhan Pişkin,Bekir Salih |
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Affiliation: | 1. The University of Akron, Department of Chemistry, Akron, OH 44325, USA;2. The Pennsylvania State University, College of Engineering, University Park, PA 16802, USA;3. Hacettepe University, Chemical Engineering Department and Bioengineering Division, and Biyomedtek: Center for Bioengineering, 06800 Ankara, Turkey;4. Hacettepe University, Department of Chemistry, 06800 Ankara, Turkey |
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Abstract: | Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an emerging technique for the determination of the molecular weight of biomolecules and their non-covalent complexes without fragmentation. One problem with this technique is the use of excess amounts of matrices, which may produce intense fragment ions and/or clusters at low mass ranges between 1 and 800 Da. These fragments lead to interference, especially concerning the signals of small target molecules. Here, a simple, reusable, and quite inexpensive approach was demonstrated to improve the effectiveness of laser desorption/ionization mass spectrometry (LDI-MS) analysis, especially for small molecules, without using matrix molecules. In this study, substrates with controllable morphologies and thicknesses were developed based on the self-assembly of silane molecules on silicon surfaces using N-(3-trimethoxysilylpropyl)diethylenetriamine (TPDA) and octadecyltrichlorosilane (OTS) molecules. Prepared substrates with nano-overlayers were successfully used in the analysis of different types of small target molecules, namely acrivastine, l-histidine, l-valine, l-phenylalanine, l-arginine, l-methionine and angiotensin I. Our substrates exhibited clear peaks almost without fragmentation for all target molecules, suggesting that these surfaces provide a number of important advantages for LDI-MS analysis, such as ease of preparation, costs, reusability, robustness, easy handling and preventing fragmentation. |
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Keywords: | LDI SAMs Matrix-free target Desorption/ionization Small molecule analysis Mass spectrometry |
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