Review of recent advances in the preparation of organic polymer monoliths for liquid chromatography of large molecules |
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| Authors: | R. Dario Arrua Mohammad Talebi Tim J. Causon Emily F. Hilder |
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| Affiliation: | 1. Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia;2. Institute for Analytical Chemistry, Johannes Kepler University, Altenberger Strasse 69, 4040 Linz, Austria |
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| Abstract: | In recent years the use of monolithic polymers in separation science has greatly increased due to the advantages these materials present over particle-based stationary phases, such as their relative ease of preparation and good permeability. For these reasons, these materials present high potential as stationary phases for the separation and purification of large molecules such as proteins, peptides, nucleic acids and cells. An example of this is the wide range of commercial available polymer-based monolithic columns now present in the market. |
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| Keywords: | AA, acrylamide ADDAB, allyldimethyldodecylammonium bromide AMPS, 2-acrylamido-2-methyl-1-propanesulfonic acid ATRP, atom transfer radical polymerization BDDMA, 1,4-butanediol dimethacrylate BMEP, bis[2-(methacryloyloxy)ethyl] phosphate BSA, bovine serum albumin BuMA, butyl methacrylate CEA, 2-carboxyethyl acrylate CEC, capillary electrochromatography CIM&trade , convective interaction media CMS, chloromethylstyrene CZE, capillary zone electrophoresis DBC, dynamic binding capacity DEA, diethylamine DMF, dimethylformamide DVB, divinylbenzene EDA, ethylenediamine EDMA, ethylene glycol dimethacrylate ESI-MS, electrospray ionization mass spectrometry GDMA, glycerol dimethacrylate GMA, glycidyl methacrylate GNPs, gold nanoparticles HDC, hydrodynamic chromatography HEMA, 2-hydroxyethyl methacrylate HIC, hydrophobic interaction chromatography HILIC, hydrophilic interaction chromatography HNPs, Hydroxyapatite nanoparticles IEX, ion-exchange chromatography LC, liquid chromatography LEDs, light emitting diodes LMA, lauryl methacrylate MAA, methacrylic acid MALDI-MS, Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry NIPA, N-isopropylacrylamide NMR, nuclear magnetic resonance PAHEMA, phosphoric acid 2-hydroxyethyl methacrylate PEG, poly(ethylene glycol) PEGA, polyethylene glycol acrylate PEGDA, polyethylene glycol diacrylate PEGDMA, polyethylene glycol dimetacrylate PEGMEA, polyethylene glycol methyl ether acrylate PLOT, porous layer open tubular POSS, polyhedral silsesquioxane RPLC, reversed-phase chromatography RSD, relative standard deviation SEC, size-exclusion chromatography SEM, scanning electron microscopy Sty, styrene TEA, triethylamine THF, tetrahydrofuran TMOS, tetramethoxysilane TOF-MS, time-of-flight mass spectrometry detection VBC, vinylbenzyl chloride VTMS, vinyltrimethoxysilane μLC, micro-liquid chromatography μLC&ndash MS/MS, micro-liquid chromatography tandem mass spectrometry |
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