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New development in in-capillary electrophoresis techniques for kinetic and inhibition study of enzymes
Authors:Hala Nehme  Reine NehmePierre Lafite  Sylvain RoutierPhilippe Morin
Affiliation:Institut de Chimie Organique et Analytique (ICOA), Université d’Orléans, CNRS FR 2708, UMR 7311, Orléans, France
Abstract:Enzymes are often quantified by measuring their biological activity. Capillary electrophoresis is gaining its position in this field due to the ongoing trend to miniaturize biochemical assays.
Keywords:BGE, background electrolyte   β-Gal, β-galactosidase   EMMA, electrophoretically mediated microanalysis   PMMA, pressure mediated microanalysis   PNPG, 4-nitro-phenyl-d-galactopyranoside   PNP, 4-nitrophenol   ONPTG, 2-nitrophenyl-1-thio-β-d-thiogalactopyranoside
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