New development in in-capillary electrophoresis techniques for kinetic and inhibition study of enzymes |
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Authors: | Hala Nehme Reine NehmePierre Lafite Sylvain RoutierPhilippe Morin |
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Affiliation: | Institut de Chimie Organique et Analytique (ICOA), Université d’Orléans, CNRS FR 2708, UMR 7311, Orléans, France |
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Abstract: | Enzymes are often quantified by measuring their biological activity. Capillary electrophoresis is gaining its position in this field due to the ongoing trend to miniaturize biochemical assays. |
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Keywords: | BGE, background electrolyte β-Gal, β-galactosidase EMMA, electrophoretically mediated microanalysis PMMA, pressure mediated microanalysis PNPG, 4-nitro-phenyl-d-galactopyranoside PNP, 4-nitrophenol ONPTG, 2-nitrophenyl-1-thio-β-d-thiogalactopyranoside |
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