Effect of the conditions of isolation on the physicochemical properties of human serum albumin in the norm and with pathology |
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Authors: | A I Ivanov R G Zhbankov E A Korolenko E V Korolik L A Meleshchenko V V Sarnatskaya V G Nikolaev V V Nikolaichik L A Yushko |
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Institution: | (1) Institute of Bioorganic Chemistry, Academy of Sciences of Belarus, Minsk;(2) B. I. Stepanov Institute of Physics, Academy of Sciences of Belarus, 70, F. Skorina Ave., 220072 Minsk, Belarus;(3) Institute of Experimental Pathology, Oncology, and Radiobiology, National Academy of Sciences of Ukraine, Kiev |
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Abstract: | Differential scanning calorimetry and IR spectrosocopy were used to investigate the effect of the procedure of isolation of
human serum albumin on its physicochemical characteristics. It is shown that fractionation of blood plasma with ethylene glycol
followed by ion exchange chromatography can be used to obtain albumin of normal donors that is similar to the albumin in the
nonfractionated plasma according to melting thermograms. Endotherms of human serum albumin samples that were obtained by affinity
chromatography and preparative electrophoresis are bimodal, unlike the monophasic for albumin obtained by polyethylene glycol
precipitation. These changes result from a higher content of nonetherified fatty acids in the albumin samples obtained by
affinity chromatography and from modification of the secondary protein structure in the samples obtained by electrophoresis.
Analysis of melting thermograms of serum albumin from patients with uremia, chronic hepatitis, and peritonitis shows that
fractionation of blood with polyethylene glycol preserves the thermodynamic characteristics of the various pathological serum
albumins to the greatest extent. The present results demonstrate the advantage of polyethylene glycol fractionation for isolation
of native preparations of normal and “pathological” human serum albumin.
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 32–37, January–February, 1997. |
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Keywords: | human serum albumin isolation methods differential scanning calorimetry IR spectroscopy |
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