Optimal speed as a function of system performance for continuous flow analyzers

The limitation to high rates of sample throughput with continuous flow automated analyzers is unacceptably high sample-to-sample carry-over. Previous programs for carry-over correction have involved relatively large computer capacities. Here, the simple expression derived for carry-over can be easily programmed in BASIC for a Commodore PET operating in real time. The program includes peak picking and calculation of the calibration graph and sample concentrations. At 240 samples per hour, the results obtained for total protein (linear calibration graph) and haptoglobin (non-linear calibration plot) showed acceptable precision and recovery and correlated well with the same determination conducted at normal operating speeds. Standard Auto Analyzer equipment was used througout. A parameter which monitors the instrument function is also calculated; this replaces the conventional visual examination of the curve for function monitoring. At high sample rates with degraded curves, visual curve examination is not effective.