Isolation,Structural Elucidation,Antioxidant and Hypoglycemic Activity of Polysaccharides of Brassica rapa L. |
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| Authors: | Wenyang Cao Chenxi Wang Xiayidan Mayhesumu Le Pan Yan Dang Abulimiti Yili Aytursun Abuduwaili Sanawar Mansur |
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| Affiliation: | 1.College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China; (W.C.); (C.W.); (X.M.); (L.P.); (Y.D.);2.Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; |
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| Abstract: | The aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of Brassica rapa L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and it obtained a polysaccharide with yield of 21.802%. Then, a neutral polysaccharide named BRP-1-1 with a molecular weight of 31.378 kDa was isolated and purified from BRP using DEAE-650 M and Sephadex G-100. The structures of the BRP-1-1 were elucidated through a combination of FT-IR, GC-MS, NMR, and methylation analysis. The results showed that BRP-1 consisted of mannose (Man) and glucose (Glu) in a molar ratio of 7.62:1. The backbone of BRP-1-1 mainly consisted of →6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→, the branch was [T-α-D-Manp-(1]n→. BRP-1-1 intervention significantly inhibited α-glucosidase activity; an inhibition rate of 44.623% was achieved at a concentration of 0.5 mg/mL. The results of the in vitro biological activity showed that BRP-1-1 has good antioxidant and hypoglycemic activity, suggesting that BRP-1-1 could be developed as a functional medicine. |
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| Keywords: | Brassica rapa L., MUAE, polysaccharides, antioxidant and hypoglycemic activities, DPPH, α -glucosidase, α -amylase |
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