Determination of d-biotin at the microgram level |
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| Authors: | Ahmed J Verma K K |
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| Institution: | Department of Chemistry, University of Jabalpur, Jabalpur 482001, India. |
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| Abstract: | The iodate formed in the reaction of d-biotin with periodate is determined by reacting it with iodide and titrating the iodine with thiosulphate (to determine 90-950 mug of biotin), or the tri-iodide is measured spectrophotometrically to determine 20-80 mug of the test compound. Excess of periodate is masked with molybdate. |
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