Colorimetric determination of dimethoate or omethoate |
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Authors: | Bhaskar S U Nandakumar N V |
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Institution: | Department of Zoology, Sri Venkateswara University, Tirupati 517502, India. |
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Abstract: | A simple, sensitive and rapid colorimetric method is described for determining dimethoate O,O-dimethyl-S-(N-methylcarbanoylmethyl)phosphorodithioate] and omethoate by an enzymatic method using pig liver acetone powder as enzyme source and p-nitrobenzenediazonium fluoroborate as the chromogenic reagent. This colorimetric method is more sensitive than non-enzymatic methods. Inhibition can be detected at ng levels and amounts ranging from 50 to 1000 ng of omethoate and from 1 to 10 mug of dimethoate can be estimated. |
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