Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules |
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Authors: | Constantin Tudor P Silva Gloria L Robertson Kelly L Hamilton Tamara P Fague Kaitlin Waggoner Alan S Armitage Bruce A |
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Affiliation: | Department of Chemistry and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA. |
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Abstract: | A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels. |
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