Simultaneous Determination of Six Major Constituents in the Stems of Kadsura heteroclita by LC-DAD |
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| Authors: | Wei Wang Jinzhi Liu Min Yang Jianghao Sun Xiaoming Wang Rongxia Liu Dean Guo |
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| Institution: | (1) Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 201203, People’s Republic of China;(2) The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, People’s Republic of China;(3) Peking University Shenzhen Hospital, Shenzhen City, 518000, Guangdong Province, People’s Republic of China |
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| Abstract: | The stem of Kadsura heteroclita is a traditional Chinese medicine with a variety of biological activities. For efficient monitoring of the quality of the herb, a simple, rapid, and accurate HPLC method has been developed for simultaneous determination of five cyclolanostane triterpenoid compounds (schisanlactone E, nigranoic acid, schisandronic acid, and heteroclitalactones A and D) and one tetrahydrofuran lignan (d-epigalbacin) in the stems of K. heteroclita. These six compounds were separated on a C18 column by elution with a mobile-phase gradient prepared from acetonitrile and 0.05% (v/v) aqueous phosphoric acid. The flow rate was 1.0 mL min−1 and the detection wavelength was 210 nm. The recovery of the method was in the range 95.03–102.19% and a good linear relationship (r
2 > 0.9999) was obtained between response and compound concentration over a relatively wide range. The method was successfully applied to analysis of the herb collected at different stages of growth. |
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| Keywords: | Column liquid chromatography Quality control Triterpenoid compounds Kadsura heteroclita |
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