| 稀土荧光螯合物时间分辨荧光光谱分析方法研究 |
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| 引用本文： | Li YH,Di YQ,Yang XY,Pan LH,Li JL,Wang YL,Li HY,Chang Y,Liu WY. 稀土荧光螯合物时间分辨荧光光谱分析方法研究[J]. 光谱学与光谱分析, 2010, 30(9): 2475-2478. DOI: 10.3964/j.issn.1000-0593(2010)09-2475-04 |
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| 作者姓名： | Li YH Di YQ Yang XY Pan LH Li JL Wang YL Li HY Chang Y Liu WY |
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| 作者单位： | 长春理工大学化学与环境工程学院,吉林,长春,130022;中国科学院长春应用化学研究所,国家电化学光谱分析研究中心,吉林,长春,130022 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 以提高固相时间分辨荧光免疫分析灵敏度为目的,在自行研制固相时间分辨荧光免疫分析样机和合成新荧光化合物的基础上,首先对新合成的4,4'-二溴-6,6'-二(N,N-二(乙氧基羰基)氨甲基)-2,2'-联吡啶-Eu(Ⅲ)螯合物的荧光性质及时间分辨荧光光谱分析方法进行了研究;其次对Eu-TTA-phen和Eu-β-NTA-TOPO等化合物进行时间分辨荧光光谱分析检测.
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| 关 键 词： | 固相时间分辨荧光光谱分析 铕螯合剂 |
Study on method of time-resolved fluorescence spectra for rare fluorescence chelate |
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| Li Yun-hui,Di Yan-qing,Yang Xiu-yun,Pan Li-hua,Li Jun-ling,Wang Yun-lei,Li Hai-yan,Chang Yu,Liu Wen-yu. Study on method of time-resolved fluorescence spectra for rare fluorescence chelate[J]. Spectroscopy and Spectral Analysis, 2010, 30(9): 2475-2478. DOI: 10.3964/j.issn.1000-0593(2010)09-2475-04 |
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| Authors: | Li Yun-hui Di Yan-qing Yang Xiu-yun Pan Li-hua Li Jun-ling Wang Yun-lei Li Hai-yan Chang Yu Liu Wen-yu |
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| Affiliation: | College of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China. liyh@cust.edu.cn |
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| Abstract: | In the present paper, the technology of time-resolved fluorescence spectra was investigated in order to improve the sensitivity of the solid-phase time-resolved fluorescence immunoassay. The fluorescence properties, fluorescence intensity and detection method of the novel complexes 4,4'-dibromo-6,6'-bis(N,N-bis(ethoxycarbonylmethyl) amino methyl)-2,2'-bipyridine were investigated through self-developed solid-phase time-resolved fluorescence immunoassay instrument. At the same time, the Eu-TTA-phen and Eu-beta-NTA-TOPO series of Eu(III) compounds were detected. |
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