Site-specific immobilization of flavin adenine dinucleotide on indium/tin oxide electrodes through flavin adenine amino group |
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Authors: | Krishna Narasimhan Lemuel B. Wingard |
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Affiliation: | (1) Department of Pharmacology, School of Medicine, University of Pittsburgh, 15261 Pittsburgh, PA |
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Abstract: | A Mannich-type reaction was used to attach flavin adenine dinucleotide (FAD) covalently to aminosilane derivatized indium/tin oxide-coated glass plates. The aminosilane was activated with formaldehyde to give an intermediate that attached specifically to the adenine amino group of FAD. The presence of the intermediate also was demonstrated by coupling hydroquinone to the formaldehyde activated support. The immobilized FAD and hydroquinone were characterized by cyclic or differential pulse voltammetry. The immobilized FAD was shown to reduce the overpotential for NADH oxidation by 180 mV. In keeping with results for FAD on glassy carbon, FAD attached to indium/tin oxide at the adenine amino group did not lead to reconstitution of activity with apoglucose oxidase. On leave from University of Madras, India. |
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Keywords: | Flavin adenine dinucleotide, immobilization on In/SnO electrodes indium/tin oxide glass immobilization of FAD on immobilized flavin, on In/SnO electrodes glucose oxidase, immobilization on In/SnO electrodes nicotinamide adenine dinucleotide, tin oxide/indium electrodes, immobilization of FAD on electrodes, immobilization of FAD on In/SnO |
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