| A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid／Liquid Interface |
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| 引用本文： | YaoQunLI JiaJuXU 等. A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid／Liquid Interface[J]. 中国化学快报, 2002, 13(6): 571-572 |
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| 作者姓名： | YaoQunLI JiaJuXU 等 |
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| 作者单位： | DepartmentofChemistryandtheKeyLaboratoryofAnalyticalSciencesofMOE,XiamenUniversity,Xiamen361005 |
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| 摘 要： | A spectrometric setup of perform total internal reflection fluorescence(TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was proposed to analyze simultaneously different components at interfaces. The TIRF excitation,emission and synchronous spectra of a water-soluble porphyrin were obtained from water/glass interface using this setup without the existence of a surfactant.
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| 关 键 词： | 固／液界面 全内反射荧光性 光谱测定装置 |
A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid/Liquid Interface |
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| Yao Qun LI,Jia Ju XU,Run Tang WANG,Li Jun YU,Zhao LI. A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid/Liquid Interface[J]. Chinese Chemical Letters, 2002, 13(6): 571-572 |
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| Authors: | Yao Qun LI Jia Ju XU Run Tang WANG Li Jun YU Zhao LI |
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| Abstract: | A spectrometric setup to perform total internal reflection fluorescence (TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was proposed to analyze simultaneously different components at interfaces. The TIRF excitation, emission and synchronous spectra of a watersoluble porphyrin were obtained from water/glass interface using this setup without the existence of a surfactant. |
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| Keywords: | Total internal reflection fluorescence synchronous fluorescence spectrofluorimeter meso-tetra(4-sulfonatophenyl)porphyrin. |
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