| 不同孔径硅胶聚合物键合相对蛋白质分离的影响 |
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| 引用本文： | 魏芸 樊立民. 不同孔径硅胶聚合物键合相对蛋白质分离的影响[J]. 分析化学, 1997, 25(9): 1043-1047 |
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| 作者姓名： | 魏芸 樊立民 |
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| 作者单位： | 中国科学院兰州化学物理研究所!兰州730000 |
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| 摘 要： | 介绍了不同孔径的硅胶聚合物键合相的合成，比较了其对蛋白质的分离特性。结果表明，大孔径的硅胶聚合物键合相比表面积小，易于获得生物大了分子的快速分离。
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| 关 键 词： | 硅胶聚合物 键合相 蛋白质 固定相 色谱 |
Effect of Different Pore Diameter Silica-based Polymer-bonded Phases on Separation of Proteins |
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| Wei Yun,Fan Limin,Chen Liren. Effect of Different Pore Diameter Silica-based Polymer-bonded Phases on Separation of Proteins[J]. Chinese Journal of Analytical Chemistry, 1997, 25(9): 1043-1047 |
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| Authors: | Wei Yun Fan Limin Chen Liren |
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| Abstract: | The preparation of macroporous silica gel matrices of different pore diameter, the synthesis of different pore diameter silica-based ploymer-bonded phaese and their chro- matographic characteristics for the separation of proteins are introduced. The results ob- trained show that macroporous packings have relatively small surface area and low capacity, and therefore benefit for the quick separation and analysis of biopolymers. The effect of salt concentration and pH value of the eluent on the retention of proteins on 300 nm silica-based polymer-bonded phase is also discussed. |
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| Keywords: | Macroporous silica gel silica-based polymer-bonded phase protein |
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