Inulinase synthesis from a mesophilic culture in submerged cultivation |
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Authors: | Ashok Pandey Simon Joseph L Ashakumary P Selvakumar Carlos R Soccol |
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Institution: | (1) Biotechnology Division, Regional Research Laboratory, Council of Scientific and Industrial Research, 695 019 Trivandrum, India;(2) Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana, CEP 81531-970 Curitiba-PR, Brazil;(3) Present address: Laboratory of Nutrition Biochemistry, National Food Research Institute, 2-1-2 Kannindai, 305 Tsukuba, Japan |
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Abstract: | A newly isolated mesophilic bacterial strain from dahlia rhizosphere, identified as Staphylococcus sp. and designated as RRL-M-5, was evaluated for inulinase synthesis in submerged cultivation using different carbon sources
individually or in combination with inulin as substrate. Inulin appeared as the most favorable substrate at a 0.5–1.0% concentration.
Media pH influenced the enzyme synthesis by the bacterial strain, which showed an optimum pH at 7.0–7.5. Supplementation of
fermentation medium with external nitrogen (organic and inorganic) showed a mixed impact on bacterial activity of enzyme synthesis.
The addition of soybean meal and corn steep solid resulted in about an 11% increase in enzyme titers. Among inorganic nitrogen
sources, ammonium sulfate was found to be the most suitable. Maximum enzyme activities (446 U/L) were obtained when fermentation
was carried out at 30°C for 24 h with a medium containing 0.5% inulin as a sole carbon source and 0.5% soybean meal as the
nitrogen source. Bacterial inulinase could be a good source for the hydrolysis of inulin for the production of d-fructose. |
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Keywords: | Inulinase mesophilic bacteria inulin submerged fermentation carbon and nitrogen sources |
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