| 肌醇与牛血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 王志军,梁瑞瑞,雷海英. 肌醇与牛血清白蛋白相互作用的荧光光谱研究[J]. 化学研究, 2012, 0(1): 64-66,101 |
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| 作者姓名： | 王志军 梁瑞瑞 雷海英 |
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| 作者单位： | [1]长治学院化学系,山西长治046011 [2]长治学院生物科学与技术系,山西长治046011 |
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| 基金项目： | 基金项目：山西省高校科技开发项目（20081042）和长治学院校级资助项目（2011116） |
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| 摘 要： | 应用荧光光谱研究了肌醇与牛血清白蛋白(BSA)分子间的相互作用;求出了猝灭常数,讨论了肌醇对BSA构象的影响,并依据能量转移理论确定了肌醇与蛋白的最近距离.结果表明,肌醇与BSA两者间的相互作用为单一的动态猝灭过程.
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| 关 键 词： | 肌醇 牛血清白蛋白 相互作用 荧光光谱 |
Fluorescence spectrometric study of the interaction between inositol and bovine serum albumin |
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| WANG Zhi-jun,LIANG Rui-rui,LEI Hai-ying. Fluorescence spectrometric study of the interaction between inositol and bovine serum albumin[J]. Chemical Research, 2012, 0(1): 64-66,101 |
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| Authors: | WANG Zhi-jun LIANG Rui-rui LEI Hai-ying |
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| Affiliation: | 1. Department of Chemistry, Changzhi University, Changzhi 046011, Shanxi, China; 2. Department of Biological Sciences and Biotechnology, Changzhi University, Changzhi 046011, Shanxi, China) |
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| Abstract: | The interaction between inositol and bovine serum albumin （BSA） at different temperature was investigated by means of fluorescence spectrometry. Quenching constant was ob- tained, and the effect of inositol on the conformation of BSA was discussed. Moreover, the shortest distance between inositol and BSA was determined based on energy transfer theory. Results show that only dynamic quenching exists between inositol and BSA. |
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| Keywords: | inositol bovine serum albumin interaction fluorescence spectrometry |
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