| 绞股蓝皂苷与牛血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 何丽君,霍彩霞,杨彩玲,魏玉荣.绞股蓝皂苷与牛血清白蛋白相互作用的荧光光谱研究[J].化学研究,2012(1):67-70. |
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| 作者姓名： | 何丽君 霍彩霞 杨彩玲 魏玉荣 |
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| 作者单位： | 兰州城市学院化学与环境科学学院,甘肃兰州730070 |
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| 基金项目： | 基金项目：甘肃省教育厅硕导项目（091113-07）. |
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| 摘 要： | 用荧光光谱技术研究了绞股蓝皂苷与牛血清白蛋白(BSA)在pH=7.40的Tris-HCl缓冲溶液中的相互作用;通过计算确定了绞股蓝皂苷与BSA的结合位点数和结合常数,利用热力学分析探讨了绞股蓝皂苷与BSA之间的结合方式;同时采用同步荧光技术考察了绞股蓝皂苷对BSA构象的影响.结果表明,绞股蓝皂苷对牛血清白蛋白的荧光猝灭过程为静态猝灭;二者主要靠疏水作用和静电引力结合.
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| 关 键 词： | 绞股蓝皂苷 牛血清白蛋白 相互作用 荧光光谱 |
Fluorescence spectrometric study of the interaction between gypenoside and bovine serum albumin |
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| HE Li-jun,HUO Cai-xia,YANG Cai-ling,WEI Yu-rong.Fluorescence spectrometric study of the interaction between gypenoside and bovine serum albumin[J].Chemical Research,2012(1):67-70. |
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| Authors: | HE Li-jun HUO Cai-xia YANG Cai-ling WEI Yu-rong |
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| Institution: | (College of Chemistry and Environmental Science, Lanzhou City University, Lanzhou 730070, Gansu, China ) |
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| Abstract: | The interaction between gypenoside and bovine serum albumin （BSA） in tris-HCl buffer solution （pH = 7.40） was studied by means of fluorescence spectroscopy. Number of binding sites and binding constant for gypenoside and BSA were determined based on calculation; and their binding modes were explored based on thermodynamic analysis. In the meantime, the effect of gypenoside on the configuration of BSA was examined by means of synchronous fluorescence spectroscopy. It was found that gypenoside quenched the fluorescence of BSA via a static quenching process, and hydrophobic and electrostatic interactions played a major role in the binding of gypenoside and BSA. |
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| Keywords: | gypenoside bovine serum albumin interaction fluorescence spectroscopy |
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