Analysis of 17β-Estadiol from Sewage in Coastal Marine Environment by Surface Plasmon Resonance Technique

In this study,surface plasmon resonance(SPR)for monitoring 17β-eatradiol(E2)was developed.The small molecule E2 was immobilized on a CM5 sensor chip for an indirect competitive immunoassay to detect E2.The SPR response based on the antigen-antibody reaction was measured by injecting the sample solution into the flow system.The limitation of detection was 0.445 μg/L.The developed SPE-SPR system was applied to analyze the seawater samples.Recovery of E2 was 91.6%-93.1%.Relative standard deviations(RSD)for the E2 assay were between 10.9%-15.1%(n=3).The range of determination of E2 samples from the sewage in the coastal marine environment was between ND(lower than detection limit)and ca.11.78 ng/L.