Influence of Freezing and Low Molecular Weight Cryoprotectants on Microsomal Membrane Structure: A Study by Multiparametric Fluorescent Probe |
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| Authors: | Tatyana S Dyubko Elena V Onishchenko Vasyl G Pivovarenko |
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| Institution: | (1) Institute for Problems of Cryobiology and Cryomedicine, Ukrainian National Academy of Sciences, 23 Pereyaslavskaya Str., Kharkov, 61015, Ukraine;(2) National Taras Shevchenko University of Kyiv, 64 Volodymyrska Str., 01033 Kyiv, Ukraine |
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| Abstract: | The influence of low molecular weight cryoprotectants (CPs) such as glycerol (GL), 1,2-propanediol (PD) and dimethylsulfoxide
(DMSO) on the structure of rat liver microsomal membranes on the stages of equilibration and upon freezing up to −196°C was
studied using a multiparametric fluorescent probe of flavonol nature. It was estimated that the studied CPs have individual
concentration ranges defining low amplitude of their action on biomembranes. An exceeding of these ranges strongly increases
the violation of membrane native structure already at the stage of incubation with CPs, strengthening it during the freezing
procedure. According to the perturbation effect on microsomal membranes the studied CPs can be arranged in a sequence: DMSO
> PD > GL. |
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| Keywords: | Microsomes Cryoprotectants Glycerol 1 2-propanediol Dimethylsulfoxide Freezing Fluorescent probe 3-hydroxyflavone Flavonol Fluorescence |
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