| 芯片毛细管电泳中组分的迁移行为及其特征 |
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| 引用本文： | 王辉,毛秀丽,盖宏伟,白吉玲,林炳承. 芯片毛细管电泳中组分的迁移行为及其特征[J]. 高等学校化学学报, 2002, 23(6): 1030-1034 |
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| 作者姓名： | 王辉 毛秀丽 盖宏伟 白吉玲 林炳承 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所, 大连 116023;2. 分子反应动力学国家重点实验室, 大连 116023 |
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| 基金项目： | 国家自然科学重点基金 (批准号 :2 0 0 3 5 0 10 )，面上基金 (批准号 :2 9975 0 3 0 )，中国科学院“寡糖工程”知识创新项目资助 |
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| 摘 要： | 在自组装的芯片毛细管电泳-激光诱导荧光检测装置上,以单个染料和一组荧光素异硫氰酸酯(FITC)标记的氨基酸为对象,研究了芯片毛细管电泳与传统毛细管电泳之间的差别,考察了玻璃芯片上微通道内的伏安特性以及抑制电压、进样方式和检测点的位置等对芯片毛细管电泳分离分析的影响,特别注意到了其有别于传统毛细管电泳的各种行为特征.
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| 关 键 词： | 芯片 激光诱导荧光 毛细管电泳 氨基酸 |
| 文章编号： | 0251-0790(2002)06-1030-05 |
| 收稿时间： | 2001-07-27 |
| 修稿时间： | 2001-07-27 |
The Behavior of Components Transfer in Chip-based Capillary Electrophoresis and Its Characteristics |
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| WANG Hui ,MAO Xiu Li ,GAI Hong Wei ,BAI Ji Ling ,LIN Bing Cheng. The Behavior of Components Transfer in Chip-based Capillary Electrophoresis and Its Characteristics[J]. Chemical Research In Chinese Universities, 2002, 23(6): 1030-1034 |
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| Authors: | WANG Hui MAO Xiu Li GAI Hong Wei BAI Ji Ling LIN Bing Cheng |
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| Affiliation: | 1. Dalian Institute of Chemical Physics, Dailan 116023, China;2. State Key Laboratory of Molecule Reaction Dynamics, Dailan 116023, China |
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| Abstract: | On the home made microchip capillary electrophoresis system with laser induced fluorescent(LIF) detection, the electrophoresis behavior of single dye and amino acids labeled with fluorescein 5 isothiocyanate(FITC) was demonstrated on glass chips. The comparison between chip based electrophoresis and conventional capillary electrophoresis was taken into account. The special attention was paid for the characteristic of chip based electrophoresis. The effect of various of operation conditions, such as countervoltage, sample loading modes and point of detection, were investigated. |
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| Keywords: | Microchip Laser induced fluorescence detection Capillary electrophoresis Amino acids |
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