Photoinitiated oxidation of NADH catalyzed by horseradish peroxidase studied by chemically induced dynamic nuclear polarization |
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| Authors: | M S Afanasyeva P A Purtov M B Taraban T V Leshina C B Grissom |
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| Institution: | (1) Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, 3 ul. Institutskaya, 630090 Novosibirsk, Russian Federation;(2) Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA |
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| Abstract: | The photoinitiated oxidation of β-NADH catalyzed by horseradish peroxidase (Per3+) was studied by time-resolved photoinitiated chemically induced dynamic nuclear polarization (CIDNP). The polarization observed
on protons at the C(4) atom of the β-NADH molecule is evidence for the reversible one-electron transfer between the radical
cation NADH+· and the ferroperoxidase intermediate (Per2+). A new approach based on electron transitions in the (NADH+· Per2+) pair was proposed to describe the formation of CIDNP effects in systems including quartet (Q)—doublet (D) electron transitions.
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 7, pp. 1090–1094, July, 2006. |
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| Keywords: | β -NADH horseradish peroxidase photoinduced one-electron transfer chemically induced dynamic nuclear polarization |
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