Automatic determination of phylloquinone in vegetables and fruits using on-line photochemical reduction and fluorescence detection via solid phase extraction and flow injection |
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Authors: | Tomás Pérez-Ruiz Carmen Martínez-Lozano Jesús Martín Maria Dolores García |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain |
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Abstract: | A very simple, rapid and highly sensitive flow injection fluorimetric method was developed for the determination of phylloquinone.
The assay was based on the on-line reduction of phylloquinone in dodecylsulfate micelles after irradiation with UV light.
The micellar medium enhanced the fluorescence and stability of the reduced phylloquinone. Under optimum experimental conditions,
the range of application of the technique was between 0.09 and 45.0 μg mL−1 and the detection limit was 0.05 μg mL−1. The sample throughput was 90 injections per hour. The reliability of the method for the routine analysis of phylloquinone
in vegetables and fruits is demonstrated. Extractions were made with hexane, and an automated solid phase extraction system
was used to purify the sample extracts prior to injection into the flow injection manifold. |
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Keywords: | Phylloquinone On-line photoreduction SDS micelles Fluorescence Flow injection Vegetables Fruits |
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