Identification of Two Palmitoyl Groups in Octopus Rhodopsin |
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| Authors: | Masashi Nakagawa Tatsuo Iwasa Satoshi Kikkawa Toshifumi Takao Yasutsugu Shimonishi Motoyuki Tsuda |
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| Institution: | Department of Life Science, Himeji Institute of Technology, Hyogo, Japan;Institute for Protein Research, Osaka University, Osaka 565, Japan |
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| Abstract: | Abstract— We determined the structure and site of fatty acid incorporated in octopus rhodopsin using a combination of fluorescence label and enzymatic cleavage methods in conjunction with fast-atom bombardment (FAB) mass spectrometry. A single peptide containing two adjacent cysteines, Cys337 and Cys338, was successfully isolated using the fluorescence from a dye conjugated to Cys345. The FAB mass spectrometric analysis of the peptide (323phe-340phe) revealed that two palmitoyl groups are linked to Cys337 and Cys338 via thioester bonds in octopus rhodopsin as in bovine rhodopsin. |
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