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Low-fluence CO2 laser irradiation decreases enamel solubility
Authors:Esteves-Oliveira  M  Apel  C  Gutknecht  N  Velloso  W F  Cotrim  M E B  Eduardo  C P  Zezell  D M
Institution:1.Centro de Lasers e Aplica??es—Instituto de Pesquisas Energéticas e Nucleares-(IPEN/CNEN-SP), S?o Paulo, SP, Brazil
;2.Department of Conservative Dentistry, Periodontology, and Preventive Dentistry, RWTH Aachen University, Aachen, Germany
;3.Basic Sciences Department, FZEA, University of S?o Paulo (USP), Pirassununga, S?o Paulo, SP, Brasil
;4.Centro de Química Ambiental-Instituto de Pesquisas Energéticas e Nucleares-(IPEN/CNEN-SP), S?o Paulo, SP, Brasil
;5.Department of Restorative Dentistry, Dentistry School of University of S?o Paulo (USP), S?o Paulo, SP, Brasil
;
Abstract:This study investigated whether subablative-pulsed CO2 laser (10.6 μm) irradiation, using fluences lower than 1 J/cm2, was capable of reducing enamel acid solubility. Fifty-one samples of bovine dental enamel were divided into three groups: control group, which was not irradiated (CG); group laser A (LA) irradiated with 0.3 J/cm2; and group laser B (LB) irradiated with 0.7 J/cm2. After irradiation, the samples were subjected to demineralization in an acetate buffer solution and were then analyzed by SEM. A finite-element model was used to calculate the temperature increase. The calcium and phosphorous content in the demineralization solution were measured with an ICP-OES. ANOVA and the t-test pairwise comparison (p < 0.016) revealed that LB showed significantly lower mean Ca and P content values in the demineralization solution than other groups. A reduction in the enamel solubility can be obtained with pulsed CO2 laser irradiation (0.7 J/cm2, 135 mJ/pulse, 74 Hz, 100 μs) without any surface photomodification and a less than 2°C temperature increase at a 3-mm depth from the surface.
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