DNA在羧基功能化吡咯共聚物导电薄膜上的固定/杂交行为

将(N-吡咯)己基硫醇自组装在金膜上,并采用化学氧化聚合法使吡咯与羧基功能化吡咯衍生物在自组装薄膜上进行共聚.然后对共聚物进行羧基活化处理.探针DNA通过与共聚物膜之间的共价键作用而固定在其表面上,接着与靶向DNA杂交.同时采用傅立叶红外变换光谱仪和X-射线光电子能谱仪对共聚物在DNA固定前后的化学组成进行详细表征.采...

DNA IMMOBILIZATION/HYBRIDIZATION ON CARBOXYL FUNCTIONALIZED PYRROLE COPOLYMER CONDUCTING FILMS

Pyrrole(Py) and pyrrole derivative with carboxyl groups,1-(2-carboxyethyl) pyrrole(PyCA),were copolymerized with 6-(1H-pyrrol-1-yl) hexane-l-thiol(PHT) self-assembled on Au surface by chemical polymerization to form a layer of the copolymer film,(poly(PHT-co-Py-co-PyCA)).The carboxyl groups of(poly(PHT-co-Py-co-PyCA)) were activated using N-(3-dimethylaminopropyl)-N′-ethylcarbodilimide(EDC) and N-hydroxysuccinimide(NHS) and transferred to amide bonds by reacting with the amino groups in the amino-labeled probe DNA.In consequence,amino-labeled DNA could be immobilized onto the copolymer.Afterwards,the complementary target DNA can be hybridized with probe DNA.The chemical structure and components of poly(PHT-co-Py-co-PyCA) before and after DNA immobilization were investigated by Fourier-transform infrared(FTIR) spectroscopy and X-ray photoelectron spectroscopy(XPS).The electrochemistry properties and the variation of the charges of copolymer film before and after DNA anchor were characterized by cyclic voltammetry and electrochemical impedance spectra.To detect the immobilization behavior of DNA,surface plasma resonance was used.The result revealed that it is a slow process for DNA anchoring onto the copolymer matrix and the electrochemical activity and the charges transfer decreased after DNA immobilization /hybridization.Furthermoret,here is no non-specific adsorption between target DNA and copolymer.It could supply the theoretical and experimental base for the application of copolypyrrole film functionalized with carboxyl groups as DNA sensors.