ISOLATION AND CHARACTERIZATION OF THE PRESUMED PHOTORECEPTOR PROTEIN OF Blepharisma japonicum |
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| Authors: | Domenico,Gioffré ,Francesco,Ghetti ,Francesco,Lenci ,Cristina,Paradiso ,Renke,Dai Pill-Soon, Song |
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| Affiliation: | CNR Istituto di Riofisica, via S. Lorenzo 26, 56127 Pisa, Italy;Department of Chemistry and Institute for Cellular and Molecular Photobiology, University of Nebraska, Lincoln, NE 68588-0304, USA |
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| Abstract: | Abstract— Photosensory responses in the ciliated protozoan Blepharisma japonicum are mediated by a hypericin-like chromophore, blepharismin, localized in granules distributed under the cell membrane. A blepharismin-binding protein, with an apparcnt molecular weight ranging between 35 and 38 kDa, has been isolated by means of column separations and preparative isoelectric focusing and characterized by means of gel electrophoresis, analytic isoelectric focusing as well as absorption and fluorescence spectroscopy. |
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