Silica-modified Fe-doped calcium sulfide nanoparticles for in vitro and in vivo cancer hyperthermia |
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Authors: | Steven Yueh-Hsiu Wu Kai-Chiang Yang Ching-Li Tseng Jung-Chih Chen Feng-Huei Lin |
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Institution: | (1) Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan;(2) Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;(3) Division of Medical Engineering Research, National Health Research Institutes, Zhunan, Taiwan;(4) National Science Council, Taipei, Taiwan, ROC; |
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Abstract: | In this study, sulfide-based magnetic Fe-doped CaS nanoparticles modified with a silica layer were investigated for cancer
hyperthermia. A polyvinyl pyrrolidone polymer was used as the coupling agent. The developed nanoparticles contained 11.6 wt%
iron concentration, and their X-ray diffraction pattern was similar to those of CaS and Fe–CaS nanoparticles. The average
particle size was approximately 47.5 nm and homogeneously dispersed in aqueous solutions. The major absorption bands of silica
were observed from the FTIR spectrum. The magnetic properties and heating efficiency were also examined. The specific absorption
ratio of nanoparticles at a concentration of 10 mg/mL at 37 °C in an ethanol carrier fluid was 37.92 W/g, and the nanoparticles
would raise the temperature to over 45 °C within 15 min. A cytotoxicity analysis revealed that the nanoparticles had good
biocompatibility, which indicated that the nanoparticles did not affect cell viability. The therapeutic effects of the nanoparticles
were investigated using in vitro and animal studies. Cells seeded with nanoparticles and treated under an AC magnetic field
revealed a percentage of cytotoxicity (60%) that was significantly higher from that in other groups. In the animal study,
during a hyperthermia period of 15 days, tumor-bearing Balb/c mice that were subcutaneously injected with nanoparticles and
exposed to an AC magnetic field manifested a reduction in tumor volume. The newly developed silica-modified Fe–CaS nanoparticles
can thus be considered a promising and attractive hyperthermia thermoseed. |
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