| 新疆紫穗槐中总黄酮的提取与含量测定 |
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| 引用本文： | 燕雪花,孙芸,刘宏炳,郑甜,田树革. 新疆紫穗槐中总黄酮的提取与含量测定[J]. 光谱实验室, 2012, 29(2): 878-881 |
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| 作者姓名： | 燕雪花 孙芸 刘宏炳 郑甜 田树革 |
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| 作者单位： | 1. 新疆名医名方与特色方剂学重点实验室,乌鲁术齐市新医路393号 830011;新疆医科大学中医学院,乌鲁木齐市新医路393号 830011
2. 新疆医科大学中医学院,乌鲁木齐市新医路393号,830011 |
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| 基金项目： | 新疆名医名方与特色方剂学实验室资助(XJDX0910-2010-07) |
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| 摘 要： | 用超声波辅助乙醇溶液浸提法和索氏提取法提取新疆地方植物药材紫穗槐中黄酮化合物并进行含量测定.以芦丁为对照品,NaNO2-Al(NO3)3-NaOH体系为显色剂,利用分光光度法在510nm处测定提取物中的总黄酮的含量.校准曲线为A=11.825C-0.0139,r=0.9992.超声提取法所得总黄酮的含量以芦丁计算为2....
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| 关 键 词： | 索氏提取法 紫穗槐 总黄酮 |
Extraction and Determination of Total Flavonoids in Amorpha fruticosa L.of Xinjiang |
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| YAN Xue-Hua , SUN Yun , LIU Hong-Bing , ZHENG Tian , TIAN Shu-Ge. Extraction and Determination of Total Flavonoids in Amorpha fruticosa L.of Xinjiang[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(2): 878-881 |
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| Authors: | YAN Xue-Hua SUN Yun LIU Hong-Bing ZHENG Tian TIAN Shu-Ge |
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| Abstract: | The total flavonoids in Amorpha fruticosa L.of Xinjiang Local medicinal plantswere compounds by Soxhlet extractor and alcohol solution extraction in cooperation with ultrasonic wave,and the content of which was determined by spectrophotometry at 510nm with rutin as standard and NaNO2-Al(NO3)3-NaOH systems as colour-developing agent.The standard curve was A=11.825C-0.0139,r=0.9992.The contents of total flavonoids in amorpha fruticosa were respectively 2.3562% and 2.6375% by the two extraction methods,and the average recoveries were 102.16% and 96.53%,respectively.Soxhelt methods is better than ultrasound-assisted alcohol extraction technology.This method is simple and accurate,and that can be applied to determine the content of total flavonoids from Amorpha fruticosa L.of Xinjiang. |
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| Keywords: | Soxhelt Methods Amorpha fruticosa L. Total Flavonoids |
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