| 分子光谱法研究硫普罗宁修饰的CdTe量子点与血红蛋白相互作用 |
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| 引用本文： | 刘丹,张惠芳,杨莉.分子光谱法研究硫普罗宁修饰的CdTe量子点与血红蛋白相互作用[J].光谱实验室,2012,29(4):2363-2367. |
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| 作者姓名： | 刘丹 张惠芳 杨莉 |
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| 作者单位： | 1. 四川文理学院化学与化学工程系 四川省达州市南坝路400号 635000
2. 西南大学化学化工学院 重庆市北碚区天生路2号 400715 |
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| 基金项目： | 四川省教育厅青年科研项目，四川文理学院重点项目 |
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| 摘 要： | 以N-(2-巯基丙酰基)-甘氨酸(硫普罗宁,TP)为稳定剂,采用水相法合成了荧光较好的水溶性CdTe量子点(TP-CdTe QDs)。本文通过共振瑞利散射光谱(RRS)、荧光光谱(FL)和紫外光谱(UV-Vis),探讨了TP-CdTe QDs与血红蛋白通过静电引力相互作用的机理.研究发现TP-CdTe QDs与血红蛋白通过静电引力相互作用以后,TP-CdTe QDs荧光猝灭发生猝灭同时荧光光谱发生蓝移,体系的共振瑞利散射光谱强度增大.血红蛋白通过静态猝灭,动态猝灭和光诱导电子转移的方式猝灭TP-CdTeQDs的荧光。同时对体系共振瑞利散射增强的原因进行了讨论。
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| 关 键 词： | TP-CdTe量子点 血红蛋白 共振瑞利散射 荧光 |
Interaction Between Tiopronin Modificatory CdTe Quantum Dots and Hemoglobin by Molecular Absorption Spectrometry |
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| LIU Dan
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ZHANG Hui-Fang
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YANG Li.Interaction Between Tiopronin Modificatory CdTe Quantum Dots and Hemoglobin by Molecular Absorption Spectrometry[J].Chinese Journal of Spectroscopy Laboratory,2012,29(4):2363-2367. |
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| Authors: | LIU Dan ZHANG Hui-Fang YANG Li |
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| Institution: | LIu Dan Zhang Hui-Fanga Yang Li (Department of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou,Sichuan 635000,P.R.China) a(School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,P.R.China) |
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| Abstract: | High quality water-soluble CdTe quantum dots(TP-CdTe QDs) were synthesized by aqueous phase method with tiopronin(TP) as stabilizer.The mechanisation of interaction between TP-CdTe QDs and hemoglobin(Hb) was investigated by resonance Rayleigh scattering(RRS) spectroscopy,fluorescence(FL) spectroscopy and ultraviolet-visible(UV-vis) absorption spectroscopy.The results indicated that the intensity of RRS of TP-CdTe QDs was obviously enhanced,while spectrum of FL was seriously quenched and blue shifted.FL of TP-CdTe QDs was quenched by Hb though static quenching,dynamic quenching and photoinduced electron transfer process.At the same time the reasons of RRS intensity enhancement of the system were discussed. |
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| Keywords: | TP-CdTe QDs Hemoglobin Resonance Rayleigh Scattering Fluorescence |
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