用傅立叶变换红外光谱法研究核辐照对虫草粉成分的影响

本文采用傅立叶变换红外光谱(卷积谱及二阶导数谱)法对经不同剂量核辐照的虫草粉进行了对比研究。虫草粉中碳水化合物(糖和纤维素)、蛋白质及脂肪含量较高; 虫草的多糖及酯对核辐照较为敏感, 辐照还使分子中吡喃环的C-O-H基团向多糖的O-H和酯的C-O-C基团转化; 构成虫草蛋白质的多肽是α构象的; 低于18 kGy剂量辐照虫草粉样品对虫草蛋白质影响很小, 但对虫草主要有效成分(含有C-N基团和N-H基团成分)影响较大; 高于18 kGy剂量辐照虫草粉样品对虫草蛋白质、多糖及酯的分子结构可能产生了破坏。建议不宜采用核辐照对虫草粉杀菌消毒处理。

A Study on the Effect of Irradiation on Components in Cordyceps Sinensis (Berk.) Saccpowder Using Fourier Transform Infrared (FTIR) Spectroscopy

FTIR spectra of Cordyceps sinensis(Berk.) Sacc powder irradiated by γ-ray with different doses were studied in the paper.Meanwhile,Fourier self-deconvolution curves and the second derivative specra for the irradiated Cordyceps sinensis(Berk.) Sacc powder were also studied.The content of carbohydrate(sugar and cellulose),protein and fat are higher in the Cordyceps sinensis(Berk.) Sacc powder;γ-ray irradiation for amylose and ester of Cordyceps sinensis(Berk.) Sacc powder is sensitiver,γ-ray irradiation for Cordyceps sinensis(Berk.) Sacc powder makes the group(C-O-H) of pyran ring of molecule toward the group(O-H) of sugar and the group(C-O-C) of ester;The polypeptide that constitute protein of Cordyceps sinensis(Berk.) Sacc are many α-configuration;It was found that the protein of the irradiated Cordyceps sinensis(Berk.) Sacc powder with the under 18 kGy had almost no changes,however,the influence of the mainly effective composition(composition of group C-N and group N-H) of Cordyceps sinensis(Berk.) Sacc powder is larger.The molecular structure of protein,amylose and cellulose of the irradiated Cordyceps sinensis(Berk.) Sacc powder with the dose of beyond 18 kGy could be broken.It is suggested that the sterilization and disinfection of Cordyceps sinensis(Berk.) Sacc powder could not use the γ-ray irradiation.