| 超高效液相色谱串联质谱技术测定麦芽中的赤霉酸 |
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| 引用本文： | 徐真,刘春凤,李永仙,郑飞云,李崎. 超高效液相色谱串联质谱技术测定麦芽中的赤霉酸[J]. 光谱实验室, 2012, 29(2): 1000-1004 |
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| 作者姓名： | 徐真 刘春凤 李永仙 郑飞云 李崎 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏省无锡市蠡湖大道1800号 214122;江南大学生物工程学院,江苏省无锡市蠡湖大道1800号 214122 |
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| 基金项目： | 国家创新基金项目(09C26213203751);江苏省创新基金项目(BC2009291);江苏高校优势学科建设工程资助项目(PAPD) |
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| 摘 要： | 利用超高效液相色谱串联质谱技术检测啤酒原料麦芽中的赤霉酸(Gibberellic acid,GA3)。样品由乙腈/水(80/20)提取后,经Oasis MAX固相萃取柱净化,以甲醇-甲酸水为流动相,梯度洗脱下,经选择离子模式(SIR)检测。结果表明,赤霉酸在ACQUITY UPLCTMBEH C18色谱柱上(2.1mm×100mm,1.7μm)得到较好分离,回收率为70%—78%,检出限0.6μg/L,RSD≤8.9%,该方法适用于麦芽等复杂体系中微量赤霉酸的检测。
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| 关 键 词： | 超高效液相色谱串联质谱 赤霉酸 麦芽 |
Determination of Gibberellic Acid in Malt by UPLC-MS |
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| XU Zhen , LIU Chun-Feng , LI Yong-Xian , ZHENG Fei-Yun , LI Qi. Determination of Gibberellic Acid in Malt by UPLC-MS[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(2): 1000-1004 |
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| Authors: | XU Zhen LIU Chun-Feng LI Yong-Xian ZHENG Fei-Yun LI Qi |
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| Affiliation: | XU Zhen LIU Chun-Feng LI Yong-Xian ZHENG Fei-Yun LI Qi(The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122,P.R.China)(School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,China) |
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| Abstract: | A method for the determination of gibberellic acid in malt was developed by ultra performance liquid chromatograph tandem mass spectrometry(UPLC-MS).Samples were extracted with acetonitrile/water(80/20),and cleaned-up with Oasis MAX solid phase extraction column.The separation was performed on an ACQUITY UPLCTM BEH C18 column(2.1mm×100mm,1.7μm),and the mobile phase was methanol /formic acid solution.Under the gradient elution,the results showed that the gibberellic acid was detected by select ion monitor,and the good separation was obtained.The recoveries were in the range of 70%—78%.The detection limit of the gibberellic acid was 0.6μg/L,and the relative standard deviations(RSDs) were less than or equal to 8.9%.The method is suitable for the determination of trace gibberellic acid in complex system as malt. |
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| Keywords: | UPLC-MS Gibberellic Acid Malt |
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