| 傅里叶变换红外光谱研究鲤鱼雌激素受体与邻苯二甲酸酯的相互作用 |
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| 引用本文： | 康丹妮,赵晓祥. 傅里叶变换红外光谱研究鲤鱼雌激素受体与邻苯二甲酸酯的相互作用[J]. 光谱实验室, 2012, 29(2): 1258-1260 |
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| 作者姓名： | 康丹妮 赵晓祥 |
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| 作者单位： | 东华大学环境科学与工程学院,上海市松江区人民北路2999号,201620 |
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| 基金项目： | 上海市基础研究重点项目(09JC1400600);上海市重点学科建设项目(B604) |
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| 摘 要： | 通过电泳结合电洗脱(SDS-PAGE)的方法分离纯化鲤鱼体内的雌激素受体,得到高纯度的受体;再通过傅里叶变换红外光谱探讨雌激素受体与邻苯二甲酸二正丁酯(DBP)结合后其结构的变化,通过实验发现,配体DBP能与雌激素受体结合并对雌激素受体的结构产生了明显的影响。
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| 关 键 词： | 邻苯二甲酸酯 雌激素受体 傅里叶变换红外光谱 |
Study on the Estrogen Receptor of Carp and Phthalic Acid Ester Interaction by Fourier Transform Infrared Spectroscopy |
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| KANG Dan-Ni , ZHAO Xiao-Xiang. Study on the Estrogen Receptor of Carp and Phthalic Acid Ester Interaction by Fourier Transform Infrared Spectroscopy[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(2): 1258-1260 |
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| Authors: | KANG Dan-Ni ZHAO Xiao-Xiang |
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| Affiliation: | KANG Dan-Ni ZHAO Xiao-Xiang(Environmental Science and Engineering,Dong Hua University,Shanghai 201620,P.R.China) |
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| Abstract: | Phthalic acid esters(PAEs) were used as plasticizers in plastic industry widely,which is one kind of environmental endocrine disruptors.They had a longer and more permanent effect on animals,they can disturb normal physiologic processes which were mediated by the estrogen receptors.The method of sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and electroelution to purify the estrogen receptor from carp,and the estrogen receptor of high purify was obtained.Fourier transform infrared spectroscopy was used to study the structure of estrogen receptor change that interact with DBP.The result prove that ligand DBP can bind with the estrogen receptor,and have an obvious effect on the structure of estrogen receptor. |
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| Keywords: | Phthalic Acid Esters Estrogen Receptor Fourier Transform Infrared Spectroscopy |
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