| 西兰花种子提取萝卜硫素的酶解体系 |
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| 引用本文： | 吴华彰,赵云利. 西兰花种子提取萝卜硫素的酶解体系[J]. 光谱实验室, 2012, 29(1): 431-434 |
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| 作者姓名： | 吴华彰 赵云利 |
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| 作者单位： | 1. 蚌埠医学院生物科学系 233030
2. 蚌埠医学院预防医学系 233030 |
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| 基金项目： | 安徽省高等学校省级教学研究项目，2011年度安徽高校自然科学研究项目 |
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| 摘 要： | 以提取萝卜硫素主要酶解因素:pH、催化缓冲液类型、Zn2+浓度、Vc的浓度为4个考察因素,分别设立3个水平,采用L9(34)正交试验设计,优化提取酶解体系.结果表明,各因素的重要性顺序为:pH＞催化缓冲液类型＞Vc的浓度＞Zn2+浓度.西兰花种子生产萝卜硫素的最佳条件为:pH 5.0、Zn2+浓度为0.1mol/L、Vc的浓度为0.5mg/mL、以0.1mol/L醋酸-醋酸钠萝卜汁缓冲液为酶解缓冲体系.验证结果显示优选的缓冲液体系高效、稳定,可以作为西兰花种子提取萝卜硫素的酶解体系.
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| 关 键 词： | 萝卜硫素 正交试验 西兰花种子 |
Enzymatic Degradation System for Sulforaphane Extraction from Broccoli Seeds |
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| WU Hua-Zhang , ZHAO Yun-Li. Enzymatic Degradation System for Sulforaphane Extraction from Broccoli Seeds[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(1): 431-434 |
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| Authors: | WU Hua-Zhang ZHAO Yun-Li |
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| Affiliation: | a (School of Biological Science,Bengbu Medical College,Bengbu,Anhui 233030,P.R.China) a(School of Prevention Medicine,Bengbu Medical College,Bengbu,Anhui 233030,P.R.China) |
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| Abstract: | In order to optimize the enzymatic degradation system for extracting sulforaphane from broccoli seeds,the L9（34） orthogonal test was used to determine the factors of pH,type of catalysis buffer,content of Zn2+ and Vc on the extraction solution,and each factor set 3 level.The important order of various factors obeyed as follows:pH > type of catalysis buffer >contentration of Vc>concentration of Zn2+.The optimum extraction conditions were pH of 5.0,0.1mol/L acetic acid-sodium acetate buffer solution with turnip juice containing 0.lmol/L Zn2+ and 0.5mg/mL Vc. The extraction buffer solution is reasonable and feasible,and can be used as enzymatic degradation for sulforaphane extraction from broccoli seeds. |
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| Keywords: | Sulforaphane Orthogonal Test Broccoli Seeds |
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