| 分光光度法测定血清铁含量 |
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| 引用本文： | 陈萍,陈俊杰,葛亚明,钟华. 分光光度法测定血清铁含量[J]. 光谱实验室, 2012, 29(3): 1645-1648 |
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| 作者姓名： | 陈萍 陈俊杰 葛亚明 钟华 |
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| 作者单位： | 1. 新乡医学院微生物学教研室 河南省新乡市金穗大道东段 453003
2. 河南科技学院动物科学学院 河南省新乡市华兰大道东段 453003 |
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| 基金项目： | 河南科技学院重点科研项目资助基金 |
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| 摘 要： | 为了达到简化操作流程、缩短测定时间的目的,本实验从样品的前处理、二氮杂菲添加量、反应时间等方面对国标测定水中铁含量的方法(二氮杂菲分光光度法,GB 8538-2008-4.15)作出改进。实验结果表明,采用干湿结合法对血清进行前处理可缩短处理时间,减少由于硝酸挥发给人体带来的伤害;二氮杂菲添加量为3mL,反应时间达14min后检测效果好,定量下限可达5mg/L。改进后测定血清铁的方法操作相对简单、检测时间短,易于推广应用。
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| 关 键 词： | 血清铁 二氮杂菲 分光光度法 |
Determination of Serum Iron Content by Spectrophotometry |
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| CHEN Ping , CHEN Jun-Jie , GE Ya-Ming , ZHONG Hua. Determination of Serum Iron Content by Spectrophotometry[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(3): 1645-1648 |
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| Authors: | CHEN Ping CHEN Jun-Jie GE Ya-Ming ZHONG Hua |
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| Affiliation: | a(Department of Microbiology,Xinxiang Medical University,Xinxiang,Henan 453003,P.R.China)a(Department of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003,P.R.China) |
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| Abstract: | In order to simplify the operation process and shorten the time of determination,the experimental aspects of sample pretreatment,the adding amount of phenanthroline,the reaction time on the determination of contents of iron in water by national standard method(Phenanthroline Spectrophotometry,GB 8538-2008-4.15)were improved.The results showed that pretreatment of serum by dry-wet combination can shorten process-time,and decrease the hurt of nitirc acid volatilization on human.There was the good detection effect when adding amount of phenanthroline was 3mL after reaction time of 14min,and the minimum detection limit can reach 5mg/L.The improved method for determination of serum iron is relatively simple,short detection time with easy application and dissemination. |
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| Keywords: | Serum Iron Phenanthroline Spectrophotometry |
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