| 稀土镧对雄性小鼠睾丸cAMP和自由基的影响 |
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| 引用本文： | 刘高峰,胡小梅,唐宝定,王春景.稀土镧对雄性小鼠睾丸cAMP和自由基的影响[J].光谱实验室,2012,29(3):1680-1683. |
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| 作者姓名： | 刘高峰 胡小梅 唐宝定 王春景 |
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| 作者单位： | 蚌埠医学院生物科学系 安徽省蚌埠市东海大道2600号 233030 |
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| 基金项目： | 安徽省教育厅自然科学基金项目，蚌埠医学院科研计划项目 |
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| 摘 要： | 采用酶联免疫吸附法和分光光度法,通过腹腔注射不同剂量稀土镧,研究小鼠睾丸环磷腺苷(cAMP)和自由基水平的变化。在稀土镧作用下,与对照组相比,处理组小鼠睾丸cAMP含量显著降低(P<0.05);MDA含量显著升高(P<0.05);GSH-PX活力无显著变化(P<0.05)。400mg·kg-1·d-1剂量组睾丸SOD活力显著降低(P<0.05)。腹腔注射稀土镧导致小鼠睾丸cAMP含量和SOD活力降低,可能是导致小鼠精子质量下降的重要原因。
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| 关 键 词： | 酶联免疫吸附法 分光光度法 镧 睾丸 环磷腺苷 自由基 |
Effects of Rare-Earth Element Lanthanum on cAMP and Free Radical in Testis of Male Mice |
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| LIU Gao-Feng
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HU Xiao-Mei
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TANG Bao-Ding
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WANG Chun-Jing.Effects of Rare-Earth Element Lanthanum on cAMP and Free Radical in Testis of Male Mice[J].Chinese Journal of Spectroscopy Laboratory,2012,29(3):1680-1683. |
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| Authors: | LIU Gao-Feng HU Xiao-Mei TANG Bao-Ding WANG Chun-Jing |
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| Institution: | LIU Gao-Feng HU Xiao-Mei TANG Bao-Ding WANG Chun-Jing(Department of Biology,Bengbu Medical College,Bengbu,Anhui 233030,P.R.China) |
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| Abstract: | Through injecting different dosages of lanthanum solution into the peritoneum of male mice,the content of testicular cAMP and testicular free radical were assayed by enzyme-linked immunosorbent assay(ELISA) and spectrophotometry.Compared with that in the control group,the content of testicular cAMP in the experimental group was significantly lower(P<0.05);the content of MDA increased significantly(P<0.05);the activity of GSH-PX had no significant difference.The activity of testicular SOD was obviously lower in the group injected 400mg·kg-1·d-1 doses(P<0.05).Rare earth lanthanum reduced the activity of SOD and the content of cAMP in testes of mice,which was probably an important reason leading to the decrease of the sperm quality of the mice. |
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| Keywords: | ELISA Spectrophotometry Lanthanum Testis cAMP Free Radical |
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