| 2,6-二(苯并咪唑-2’)吡啶的抗氧化活性及其与BSA和DNA相互作用的光谱研究 |
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| 引用本文： | 凌钦婕,招振楠,李林林,黄峙,杨芳,郑文杰.2,6-二(苯并咪唑-2’)吡啶的抗氧化活性及其与BSA和DNA相互作用的光谱研究[J].光谱实验室,2012,29(4):2321-2326. |
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| 作者姓名： | 凌钦婕 招振楠 李林林 黄峙 杨芳 郑文杰 |
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| 作者单位： | 1. 暨南大学生物工程学系 广州市黄埔大道601号 510632
2. 暨南大学化学系 广州市黄埔大道601号 510632 |
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| 基金项目： | 科技部国家科技支撑计划，教育部博士点基金 |
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| 摘 要： | 采用光谱法研究了2,6-二(苯并咪唑-2’)吡啶(bbp)的抗氧化活性,及其与牛血清白蛋白(BSA)和DNA的相互作用。实验结果表明,bbp能有效清除ABTS自由基,具有较好的抗氧化活性;bbp与BSA相互作用,改变了血清蛋白的空间构象,同时引起酪氨酸残基所处微环境极性的变化;通过粘度实验验证了bbp能以插入方式与DNA结合。实验结果对揭示bbp在药物化学应用中的作用机理提供重要信息。
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| 关 键 词： | 2 6-二(苯并咪唑-2’)吡啶 牛血清白蛋白 脱氧核糖核酸 紫外-可见分光光度法 荧光分光光度法 |
Antioxidant Activity of 2,6-Bis (Benzimidazol-2-Yl)Pyridine and Spectra Study on Its Interaction with Bovine Serum Albumin and DNA |
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| LING Qin-Jie
,
ZHAO Zhen-Nan
,
LI Lin-Lin
,
HUANG Zhi
,
YANG Fang
,
ZHENG Wen-Jie.Antioxidant Activity of 2,6-Bis (Benzimidazol-2-Yl)Pyridine and Spectra Study on Its Interaction with Bovine Serum Albumin and DNA[J].Chinese Journal of Spectroscopy Laboratory,2012,29(4):2321-2326. |
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| Authors: | LING Qin-Jie ZHAO Zhen-Nan LI Lin-Lin HUANG Zhi YANG Fang ZHENG Wen-Jie |
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| Institution: | Ling Qin-Jie Zhao Zhen-Nana LI Lin-Lina Huang Zhi Yang Fanga Zheng Wen-Jiea (Department of Biology,Jinan University,Guangzhou 510632,P.R.China) a(Department of Chemistry,Jinan University,Guangzhou 510632,P.R.China) |
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| Abstract: | The antioxidant activity of 2 > 6-bis(benzimidazol-2-yl) pyridine(bbp) and its interaction with bovine serum albumin(BSA) and calf thymus DNA were investigated by spectroscopy.The results showed that bbp could effectively scavenge the ABTS free radicals, demonstrating its strong antioxidant activity,bbp could cause the change in the configuration of BSA through interaction with tyrosine residue,leading to changes in the tyrosine residues microenvironment polarity.Viscosity exprimental results also showed that bbp could bind to DNA in the form of insert mode.This study could provide important information for elucidation of the action mechanisms of bbp in medicinal chemistry. |
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| Keywords: | bbp BSA DNA UV-Vis Spectrophotometry Fluorescence Spectrophotometry |
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