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| 紫外可见吸收光谱检测pH值对光聚合脂质体的影响 | |
| 引用本文： | 郭讯枝,何剪太,张阳德,周伟华. 紫外可见吸收光谱检测pH值对光聚合脂质体的影响[J]. 光谱实验室, 2012, 29(1): 506-510 |
| 作者姓名： | 郭讯枝 何剪太 张阳德 周伟华 |
| 作者单位： | 1. 中南大学卫生部肝胆肠外科研究中心生物医学工程研究院 410008;宜春学院江西省天然药物活性成分研究重点实验室 336000 2. 中南大学卫生部肝胆肠外科研究中心生物医学工程研究院 410008 3. 宜春学院江西省天然药物活性成分研究重点实验室 336000 |
| 基金项目： | 江西省教育厅科技项目，江西省科技支撑计划 |
| 摘 要： | 利用紫外可见吸收光谱法考察脂质溶液pH值对光聚合脂质体的影响.通过水浴超声法制备光聚合脂质体,然后以脂质体的光学特性作为主要指标,利用光谱扫描测量各体系在640nm、540nm处的吸光值,利用比色响应值(CR)来对不同pH值下光聚合脂质体的颜色变化进行定量分析.结果表明,随着pH值的增大,光聚合脂质体溶液颜色由蓝色逐渐变成深红色. |
| 关 键 词： | 脂质体 光聚合 紫外可见吸收光谱 比色响应值 |
Detection of Influence of pH Value on Photopolymerization Liposomes by UV-Visible Absorption Spectrum |
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| GUO Xun-Zhi , HE Jian-Tai , ZHANG Yang-De , ZHOU Wei-Hua. Detection of Influence of pH Value on Photopolymerization Liposomes by UV-Visible Absorption Spectrum[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(1): 506-510 | |
| Authors: | GUO Xun-Zhi HE Jian-Tai ZHANG Yang-De ZHOU Wei-Hua |
| Affiliation: | GUO Xun-Zhi(a,b) He Jian-Taia Zhang Yang-Dea Zhou Wei-Huab a(National Hepatobiliary & Enteric Surgery Research Center,Ministry of Health,Institute of Biomedical Engineering of Central South University,Changsha 410008,P.R.China) b(Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun University, Yichun.Jiangxi 336000,P.R.China.) |
| Abstract: | The influence of pH value of the lipid solution on photopolymerization liposomes was investigated by UV-Visible absorption spectrometry.The photopolymerization liposomes were prepared by water bath ultrasound method with optical properties of liposomes as main index,and the absorbance of each system in wavelength of 640nm and 540nm was determined by spectrum scan,and color changes of the photopolymerization liposomes under different pH value were quantitatively analyzed by colorimetric response.The results show that the color of photopolymerization liposome solution gradually changes from the blue color into the deep red with the increase of the pH value. |
| Keywords: | Lipsome Photopolymerization UV-Visible Absorption Spectrum CR(Colorimetric Response) |
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