| HPLC-MS/MS检测人血浆中的茴三硫代谢物对羟基茴三硫及生物等效性 |
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| 引用本文： | 王晓东,郝卫强,吴荷琴,陈强.HPLC-MS/MS检测人血浆中的茴三硫代谢物对羟基茴三硫及生物等效性[J].光谱实验室,2012,29(4):2506-2509. |
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| 作者姓名： | 王晓东 郝卫强 吴荷琴 陈强 |
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| 作者单位： | 1. 常州四药制药有限公司技术中心 江苏省常州市离宫路168号 213004
2. 南京大学常州高新技术研究院分离实验室 江苏省常州市常武中路801号 213164 |
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| 基金项目： | 国家自然科学基金，常州市应用基础研究计划 |
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| 摘 要： | 用HPLC-MS/MS内标法定量测定人血浆中茴三硫代谢物对羟基茴三硫的浓度。采用InertsilODS-3色谱柱(150mm×2.1mm,3μm),流动相为甲醇;水=85:15(V/V),采用多反应监测(MRM)模式。对羟基茴三硫在0.452—603.2ng/mL浓度范围内具有良好的线性关系,日内、日间相对标准偏差(RSD)和准确度误差(RE)均小于12%。该方法灵敏、准确、快速,已用于茴三硫片体内药动学的研究。
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| 关 键 词： | 高效液相色谱-串联质谱法 血浆 茴三硫 对羟基茴三硫 相对生物利用度 |
Determination of p-Hydroxyanetholtrithion in Human Plasma by HPLC-MS/MS and Its Bioequivalence |
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| WANG Xiao-Dong
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HAO Wei-Qiang
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WU He-Qin
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CHEN Qiang.Determination of p-Hydroxyanetholtrithion in Human Plasma by HPLC-MS/MS and Its Bioequivalence[J].Chinese Journal of Spectroscopy Laboratory,2012,29(4):2506-2509. |
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| Authors: | WANG Xiao-Dong HAO Wei-Qiang WU He-Qin CHEN Qiang |
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| Institution: | Wang Xiao-Dong Hao Wei-Qianga Wu He-Qin Chen Qianga (Changzhou Siyao Pharmaceuticals Co.,Ltd,Changzhou,Jiangsu 213004,P.R.China) a(High-Tech Research Institute of Nanjing University,Changzhou,Jiangsu 213164,P.R.China) |
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| Abstract: | The concentration of p-hydroxyanetholtrithion(HAT) in human plasma was determined by HPLC-MS/MS using internal standard method.The separation was carried out by using Inertsil ODS-3 column(150mm×2.1mm,3μm) as stationary phase,methanol:water=85:15 (V:V)as mobile phase,and multiple reaction monitoring(MRM) mode for detection.There was a good linear relationship when concentration of HAT was in the range of 0.452-603.2ng/mL.The relative standard deviation(RSD) of inter-day and intra-day and the relative error(RE) of accuracy were all less than 12%.This method is sensitive,accurate and fast,and has been applied to the pharmacokinetic study of anetholtrithion tablets. |
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| Keywords: | HPLC-MS/MS Plasma Anetholtrithion p-Hydroxyanetholtrithion Relative Bioavailability |
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