| 分散液相微萃取-高效液相色谱法测定蜂蜜中的磺胺类抗生素 |
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| 引用本文： | 包锦渊,迟晓峰,董琦,胡凤祖.分散液相微萃取-高效液相色谱法测定蜂蜜中的磺胺类抗生素[J].光谱实验室,2012,29(3):1934-1937. |
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| 作者姓名： | 包锦渊 迟晓峰 董琦 胡凤祖 |
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| 作者单位： | 1. 青海民族大学化学与生命科学学院 西宁市八一中路72号 810000
2. 中国科学院西北高原生物研究所 西宁市新宁路23号 810008 |
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| 摘 要： | 应用分散液相微萃取(DLLME)技术,建立了蜂蜜中磺胺类抗生素的高效液相色谱分析方法。考察了萃取剂、分散剂、萃取剂体积、分散剂体积、时间、盐度和pH等因素对分散液相微萃取的影响,并确定了最佳萃取条件为:40μL 1,1,2,2-四氯乙烷(萃取剂)和1mL乙腈(分散剂),混匀后,加入水样,离心2min,吸取5μL沉积相,进行HPLC分析。应用本方法对3批蜂蜜样品进行了测定,取得了良好的效果。
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| 关 键 词： | 分散液相微萃取 高效液相色谱 磺胺 蜂蜜 |
Determination of Sulfonamides in honey by DLLME-HPLC |
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| BAO Jin-Yuan
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CHI Xiao-Feng
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DONG Qi
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HU Feng-Zu.Determination of Sulfonamides in honey by DLLME-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2012,29(3):1934-1937. |
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| Authors: | BAO Jin-Yuan CHI Xiao-Feng DONG Qi HU Feng-Zu |
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| Institution: | BAO Jin-Yuan CHI Xiao-Feng a DONG Qi a HU Feng-Zu a(Department of Chemistry and Life Science,Qinghai University for Nationalities,Xining 810000,P.R.China)a(Northwest Institute of Plateau Biology of Chinese Academy of Sciences,Xining 810008,P.R.China) |
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| Abstract: | The method for analysis of sulfonamides antibiotic in honey by dispersive liquid-liquid microextraction(DLLME) combined with high performance liquid chromatography was developed.The effects of parameters such as the extraction solvent,dispersing agent,volume of extraction solvent and dispersing solvent,extraction time,ionic strength and solution pH on dispersion of liquid phase microextraction were investigated,and the optimal extraction conditions were as follows:1mL of acetonitrile(as dispersive solvent) and 40μL 1,1,2,2-tetrachloroethane(as extraction solution) were rapidly mixed,then centrifuged for 2min after adding water solution of honey samples,and 5μL sedimentary facies was injected to analyze by HPLC.The method is applied to determination of 3 batches honey samples with good effects. |
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| Keywords: | Dispersive Liquid-Liquid Microextraction HPLC Sulfonamides Honey |
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