| 荷移分光光度法测定氧氟沙星及其分析应用 |
| |
| 引用本文： | 孙雪花,马红燕,高楼军,齐广才,张蒙.荷移分光光度法测定氧氟沙星及其分析应用[J].光谱实验室,2012,29(2):1022-1024. |
| |
| 作者姓名： | 孙雪花 马红燕 高楼军 齐广才 张蒙 |
| |
| 作者单位： | 延安大学化学与化工学院,陕西省延安市,716000 |
| |
| 基金项目： | 陕西省延安市科技局基金项目(2009kg-02) |
| |
| 摘 要： | 研究了氧氟沙星与茜素红在水系中发生的荷移反应。确定了最佳反应条件,建立了一种快速简便测定氧氟沙星的荷移分光光度法。结果表明,荷移络合物的最大吸收波长是522nm,表观摩尔吸光系数是8.64×103L.mol-1.cm-1,络合比为1∶1,氧氟沙星的浓度在0.5—25mg/L范围内服从比耳定律,相对标准偏差为0.8%,检出限为0.38mg/L。用于氧氟沙星片剂含量的测定,回收率在97.0%—101.0%以上。此方法简单、灵敏度较高、选择性较强,结果令人满意。
|
| 关 键 词： | 荷移反应 茜素红 氧氟沙星 分光光度法 |
Determination of Ofloxacin by Charge Transfer Spectrophotometry and Its Analytical Application |
| |
| SUN Xue-Hua
,
MA Hong-Yan
,
GAO Lou-Jun
,
QI Guang-Cai
,
ZHANG Meng.Determination of Ofloxacin by Charge Transfer Spectrophotometry and Its Analytical Application[J].Chinese Journal of Spectroscopy Laboratory,2012,29(2):1022-1024. |
| |
| Authors: | SUN Xue-Hua MA Hong-Yan GAO Lou-Jun QI Guang-Cai ZHANG Meng |
| |
| Institution: | SUN Xue-Hua MA Hong-Yan GAO Lou-Jun QI Guang-Cai ZHANG Meng(College of Chemistry and Chemical Engineering,Yan’an University,Yan’an,Shaanxi 716000,P.R.China) |
| |
| Abstract: | The charge transfer reaction in water between ofloxacin and alizarin red was investigated by spectrophotometry.The experimental optimum conditions were obtained,and a simple and rapid method for determination of ofloxacin was developed.The results showed that the maximum absorption wavelength of charge transfer complex was 522nm,molar absorption coefficient was 8.64×103L·mol-1·cm-1,and complex ratio was 1∶1.The concentration of ofloxacin obeyed Beer law in the range of 0.5—25mg/L.The relative standard deviation was 0.8%.The detection limit was 0.38mg/L,and the recovery was in the range of 97.0%—101.0%.This method is simple,high sensitive and strong selective with satisfactory results. |
| |
| Keywords: | Charge Transfer Reaction Alizarin Red Ofloxacin Spectrophotometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
