| 高效液相色谱-电喷雾/质谱法测定青稞幼苗提取物氨基酸 |
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| 引用本文： | 王萍,陈涛,杜玉枝,沈裕虎,李玉林.高效液相色谱-电喷雾/质谱法测定青稞幼苗提取物氨基酸[J].光谱实验室,2012,29(3):1351-1355. |
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| 作者姓名： | 王萍 陈涛 杜玉枝 沈裕虎 李玉林 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所 西宁市新宁路23号 810008;中国科学院研究生院 北京市石景山区玉泉路19号甲 100049
2. 中国科学院西北高原生物研究所 西宁市新宁路23号 810008 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目 |
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| 摘 要： | 以2-2-(7H-二苯并a,g]咔唑-乙氧基)-乙基]氯甲酸酯(DBCEC-Cl)作为柱前衍生试剂,采用高效液相色谱-电喷雾/质谱法(HPLC-ESI/MS)对青稞幼苗提取物中的氨基酸进行了测定。结果表明,青稞幼苗提取物中含有13种氨基酸,总含量为81.6mg/g,其中有人体自身不能合成的6种重要氨基酸赖氨酸(Lys)、蛋氨酸(Met)、亮氨酸(Leu)、异亮氨酸(Ile)、苏氨酸(Thr)和缬氨酸(Val)],含量达41.8mg/g,占总氨基酸的51.2%。该方法灵敏、可靠、重现性好,是一种理想的全谱氨基酸分析方法。
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| 关 键 词： | 青稞 氨基酸 高效液相色谱-电喷雾/质谱法 2-[2-(7H-二苯并[a g]咔唑-乙氧基)-乙基]氯甲酸酯 |
Determination of Amino Acids from Highland Barley Seedlings Extract by HPLC-ESI/MS |
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| WANG Ping
,
CHEN Tao
,
DU Yu-Zhi
,
SHEN Yu-Hu
,
LI Yu-Lin.Determination of Amino Acids from Highland Barley Seedlings Extract by HPLC-ESI/MS[J].Chinese Journal of Spectroscopy Laboratory,2012,29(3):1351-1355. |
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| Authors: | WANG Ping CHEN Tao DU Yu-Zhi SHEN Yu-Hu LI Yu-Lin |
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| Institution: | a a(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,P.R.China)b(Graduate School of Chinese Academy of Sciences,Beijing 100049,P.R.China) |
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| Abstract: | The amino acids in highland barley seedlings extract was determined by HPLC-ESI/MS with 2-2-(dibenzocarbazole)-ethoxy] ethyl chloroformate(DBCEC-Cl) as the sensitive pre-column derivatization reagent.The results showed that there were 13 kind of amino acids in highland barley seedlings extract,and the total content was 81.6mg/g,and there were 6 kinds of amino acids that couldn’t be synthesized by the body itself,including lysine(Lys),methionine(Met),leucine(Leu),isolencine(Ile),threonine(Thr) and valine(Val),and their contents amounted to 41.8mg/g with accounting for 51.2% of the total amino acids.The method is sensitive and reliable with good repeatability,that is as the ideal analysis of full-spectrum amino acids in highland barely seedlings. |
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| Keywords: | Highland Barley Amino Acids HPLC-ESI/MS DBCEC-Cl |
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