| 荧光探针辅助研究硒(Ⅲ)、土霉素与DNA的相互作用 |
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| 引用本文： | 魏光成,付彩霞,闫苗苗. 荧光探针辅助研究硒(Ⅲ)、土霉素与DNA的相互作用[J]. 光谱实验室, 2012, 29(2): 865-869 |
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| 作者姓名： | 魏光成 付彩霞 闫苗苗 |
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| 作者单位： | 滨州医学院药学院,山东省烟台市莱山区观海路346号,264003 |
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| 基金项目： | 山东省自然科学基金(ZR2010BL026);山东省高校科技计划(J09LC66) |
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| 摘 要： | 采用偶氮胭脂红(G)荧光探针作为辅助手段,利用紫外-可见及荧光光谱技术分别测定了土霉素、硒(Se2+)与DNA的相互作用的光谱,得到了土霉素-硒离子与DNA的作用方式为嵌插作用,土霉素-硒离子与DNA的相互作用的固有结合常数K为1.03×104L/mol。
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| 关 键 词： | 荧光光谱 DNA 荧光探针 土霉素 硒离子 |
Interactions of Selenium Ions,Oxytetracycline and DNA by Fluorescence Probe |
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| WEI Guang-Cheng , FU Cai-Xia , YAN Miao-Miao. Interactions of Selenium Ions,Oxytetracycline and DNA by Fluorescence Probe[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(2): 865-869 |
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| Authors: | WEI Guang-Cheng FU Cai-Xia YAN Miao-Miao |
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| Affiliation: | WEI Guang-Cheng FU Cai-Xia YAN Miao-Miao(Department of Pharmacy,Binzhou Medical Colleage,Yantai,Shandong 264003,P.R.China) |
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| Abstract: | Using azocarmine(G) fluorescence probe as supplementary means,and the interaction spectra of Se2+,oxytetracycline with DNA was determined by UV-Visible spectrum and fluorescence spectrum.The interaction mode of oxytetracycline-Se2+ with DNA was intercalative,and the conjugated constant of oxytetracycline-Se2+ with DNA was 1.03×104L/mol. |
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| Keywords: | Fluorescence Spectrometry DNA Fluorescence Probe Oxytetracycline Selenium Ions |
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