Simultaneous Determination of Phytohormones in Plant Extracts using SPME and HPLC |
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Authors: | Hong-Tao Liu Yan-Fen Li Tian-Gang Luan Chong-Yu Lan Wen-Sheng Shu |
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Institution: | (1) School of Life Sciences, State Key Laboratory of Biocontrol, Sun Yat-Sen (Zhongshan) University, Guangzhou, 510275, China |
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Abstract: | Solid-phase microextraction followed by HPLC was used for the determination of indole-3-acetic acid (IAA), abscisic acid (ABA),
indole-3-butyric acid (IBA) and 1-naphthylacetic acid (NAA) in plant samples. Parameters influencing performance, including
pH, salinity, extraction time, fiber coating and temperature, were optimized. A Carbowax-coated fiber was chosen for determination
due to much higher extraction efficiency compared to polyacrylate fibers. The dynamic ranges spanned over three orders of
magnitude. The LOD/(LOQ) values of the target compounds in pure water were 0.149(0.497), 0.442(1.472), 0.121(0.403), 0.058(0.193)
μg L−1 for IAA, ABA, IBA and NAA respectively. The method was successfully applied to the analysis of xylem fluid from Musa basjoo stem obtaining recoveries of 98.85% (IAA), 94% (IBA) and 94.30% (NAA). The method was also successfully applied to the analysis
of these four target compounds in the hyperaccumulating plant, Viola baoshanensis. The results matched quite well with ones obtained by solid phase extraction followed by HPLC. The method developed was superior
when applied to liquid samples because matrix effects could be eliminated. |
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Keywords: | Column liquid chromatography Solid-phase microextraction (SPME) Phytohormones Viola baoshanensis and Musa basjoo Xylem fluid |
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