Gold nanoparticle-based signal augmentation of quartz crystal microbalance immunosensor measuring C-reactive protein |
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Affiliation: | Food Bio-nano Technology Research Group, Korea Food Research Institute, Songnam 463-746, Republic of Korea |
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Abstract: | A biotinylated anti-rat C-reactive protein (CRP) antibody was ingeniously prepared by the reaction of the unmodified antibody with a water-soluble sulfosuccinimidyl-6-(biotinamido)hexanoate. The molar biotin incorporation of the resulting modified antibody was found as 5.82. A flow-type indirect-competitive quartz crystal microbalance immunosensor system was constructed with the sensor chip immobilized with 2 mg/mL CRP as the coating antigen. When 200 μL of the modified antibody having the concentration of 0.250 mg/mL was added with a streptavidin-coated gold nanoparticle (GNP) to the immunosensor system, the frequency shift obtained was 139.8 ± 0.3 Hz. Compared to the frequency shift of 91.1 ± 1.3 Hz found with the addition of the unmodified antibody only, the signal augmentation after GNP binding amounted to 53.4%, which resulted in sensitivity improvement of the current immunosensor. |
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