Simultaneous Quantification of 3′- and 6′-Sialyllactose in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study |
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| Authors: | Seok-In Jang Han Young Eom Jeong Ho Hwang Lila Kim Jong-Hwa Lee |
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| Institution: | 1.Bioanalytical and Pharmacokinetic Study Group, Korea Institute of Toxicology, Daejeon 34114, Korea; (S.-I.J.); (H.Y.E.);2.Animal Model Research Group, Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup, Jeollabuk-do, Daejeon 53212, Korea;3.GeneChem Inc., A-501, 187 Techno 2-ro, Daejeon 34025, Korea; |
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| Abstract: | Sialyllactose (SL), an acidic oligosaccharide, has immune-protective effects against pathogens and helps with the development of the immune system and intestinal microorganisms. To elucidate the pharmacokinetic characterization after oral administration to rats, the simultaneous quantification method for 3′-SL and 6′-SL in rat plasma was validated, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in an electrospray ionization (ESI) mode. Several types of columns C18, amide, and hydrophilic interaction liquid chromatography (HILIC) phase] were used to separate the peaks of 3′-SL and 6′-SL, which improved chromatographic selectivity. Ultimately, the HILIC phase column had a good peak shape and quick resolution, with a mobile phase comprising ammonium acetate buffer and acetonitrile obtained by gradient elution. In addition, the simultaneous quantification of 3′-SL and 6′-SL in rat plasma samples were adequately applied to pharmacokinetic study. |
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| Keywords: | sialyllactose method validation HILIC column mass spectrometry surrogate matrix |
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