High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents |
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Authors: | Dueymes Cécile Schönberger Andreas Adamo Ilaria Navarro Aude-Emmanuelle Meyer Albert Lange Meinolf Imbach Jean-Louis Link Fritz Morvan François Vasseur Jean-Jacques |
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Affiliation: | ERT Oligonucléotides: Méthodologie, Valorisation, UMR 5625 CNRS-UM2 University Montpellier II, 34095 Montpellier, France. |
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Abstract: | A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text] |
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