| DNA在碳纳米管表面的固定、表征及损伤的电化学检测 |
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| 引用本文： | 刘姝娜,吴萍,周泊,周耀明,蔡称心.DNA在碳纳米管表面的固定、表征及损伤的电化学检测[J].化学学报,2008,66(4):424-432. |
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| 作者姓名： | 刘姝娜 吴萍 周泊 周耀明 蔡称心 |
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| 作者单位： | (南京师范大学化学与环境科学学院 生物功能材料江苏省重点实验室分子医学生物技术江苏省重点实验室 南京 210097) |
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| 基金项目： | 国家自然科学基金
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教育部跨世纪优秀人才培养计划
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江苏省自然科学基金
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江苏省实验室基金
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江苏省高校自然科学基金 |
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| 摘 要： | 将DNA通过PDDA poly(dimethyldiallylammonium chloride)]固定在单壁碳纳米管(CNT)表面, 形成DNA-PDDA-CNT纳米复合体, 用AFM、UV-Vis、Raman光谱及交流阻抗对其进行了表征. 用伏安法研究了1-苯基偶氮-2-萘酚(PN)与固定在CNT表面的DNA的相互作用, 结果表明PN与DNA的作用方式为嵌入作用；并且, PN能用作电化学检测DNA化学损伤的探针分子. 本文电化学检测DNA损伤的优点在于PN的氧化还原式量电位E0'≈0.1 V (vs. SCE, pH 5.5), 能有效降低其它电活性物质对DNA损伤电化学检测的干扰.
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| 关 键 词： | 碳纳米管 DNA DNA损伤 电化学检测 |
| 收稿时间： | 2007-07-11 |
| 修稿时间： | 2007-10-30 |
Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage |
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| LIU Shu-Na,WU Ping,ZHOU Bo,ZHOU Yao-Ming,CAI Chen-Xin.Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage[J].Acta Chimica Sinica,2008,66(4):424-432. |
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| Authors: | LIU Shu-Na WU Ping ZHOU Bo ZHOU Yao-Ming CAI Chen-Xin |
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| Institution: | (College of Chemistry and Environmental Science, Jiangsu Key Laboratory for Biofunctional Materials, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing 210097) |
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| Abstract: | DNA-PDDA-CNT nanocomposites were fabricated by immobilizing DNA on the surface of single-walled carbon nanotubes (CNT) via poly(dimethyldiallylammonium chloride) (PDDA). The atomic force microscopy (AFM), ultraviolet-visible (UV-Vis), Raman spectroscopy and impedance spectroscopy (EIS) were used to characterize the growth processes of the nanocomposites. The cyclic voltammetric results indicate that the interaction between 1-phenylazo-2-naphthol (PN) and the immobilized DNA is intercalative binding, and PN can be used as a probe for detection of DNA damage. PN seems to be an attractive probe for DNA damage analysis since its redox peak is at ca. 0.1 V (vs. SCE, pH 5.5), where interference should be minimal. |
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| Keywords: | carbon nanotube DNA DNA damage electrochemical detection |
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