Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection |
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| Authors: | Ajeet Kaushik Pratima R. Solanki Keiichi Kaneto C. G. Kim Sharif Ahmad Bansi D. Malhotra |
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| Affiliation: | 1. Department of Science and Technology Centre on Biomolecular Electronics, National Physical Laboratory, New Delhi 110012, India;2. Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India;3. Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu, 808‐0196, Japan;4. Centre for Nano‐Bioengineering and Spintronics, Chungnam National University Daejeon, 305‐764, Korea |
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| Abstract: | A nanostructured iron oxide (NanoFe3O4, particle size ca. 25 nm and roughness ca. 21 nm) film deposited onto a hydrolyzed indium‐tin‐oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe3O4 nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe3O4/ITO bioelectrode exhibit improved linearity (2.5–400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Ω/mg dL?1/cm?2) and a low value of the Michaelis‐Menten constant (Km, 0.8 mg/dL) with a regression coefficient of 0.997. |
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| Keywords: | Nanostrutured Fe3O4 Impedimetric biosensors Cholesterol oxidase Biosensors |
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