A Comparison of Membrane Inlet Mass Spectrometry and Nitric Oxide (NO) Electrode Techniques to Detect NO in Aqueous Solution |
| |
| Authors: | Chingkuang Tu Nikki Scafa Xueji Zhang David N Silverman |
| |
| Institution: | 1. Department of Pharmacology, University of Florida College of Medicine, Gainesville, FL 32610‐0267, USA;2. World Precision Instruments, Inc., Sarasota International Trade Center, 175 Sarasota Center Boulevard, Sarasota, FL 34234‐9258, USA |
| |
| Abstract: | The NO electrode and membrane inlet mass spectrometry (MIMS) have the advantage of being sensitive, direct, and real time detectors of NO in aqueous solution. They do not require reacting NO with labels or purging of NO with an inert gas. We show that the NO electrode and MIMS are comparable in sensitivity detecting NO concentrations to 0.5 nM in aqueous solution, and both give identical results in a biological measurement, the reactions of deoxyhemoglobin with nitrite. |
| |
| Keywords: | NO electrode Membrane inlet Mass spectrometry Nitrite Hemoglobin |
|
|
