An L‐Lactate Amperometric Enzyme Electrode Based on L‐Lactate Oxidase Immobilized in a Laponite Gel on a Glassy Carbon Electrode. Application to Dairy Products and Red Wine |
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| Authors: | Veronica?Paz Zanini Beatriz?López de?Mishima Pierre Labbé Velia Solís |
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| Institution: | 1. INQUINOA‐CONICET, Instituto de Ciencias Químicas, Facultad de Agronomía y Agroindustrias, Universidad Nacional de Santiago del Estero, Belgrano (S) 1912, 4200 Santiago del Estero, Argentina;2. Département de Chimie Moléculaire, UMR CNRS 5630, Université Joseph Fourier, BP 53, 38041 Grenoble cedex 09, France;3. INFIQC‐CONICET, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. Haya de la Torre s/n, Ciudad Universitaria, 5000 Córdoba, Argentina |
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| Abstract: | A biosensor based on the immobilization of Lactate oxidase in laponite–organosilasesquioxane films on glassy carbon electrode for the quantification of L ‐lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33±0.01) A cm?2 M?1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our L ‐lactate determinations were in good agreement with those of two standard methods. |
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| Keywords: | Lactate oxidase biosensor Laponite gel L‐lactate Ferrocene‐methanol Wine Dairy products Biosensors Enzymes |
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